ABSTRACT
Objectives: To study the prevalence of HBsAg, Anti-HCV, HIV, Syphilis and Malaria in blood donors
Methods: This is a cross sectional descriptive study, conducted at Blood bank and Transfusion center at Liaquat University of Medical and Health Sciences [LUMHS] Hyderabad, during the period from January, 2014 to June, 2015.A total of 4683 blood donors were screened for HBsAg, Anti-HCV and HIV on Architect 20001 [manufactured by Abbott], employing chemiluminescent microparticle immunoassay [CMIA]. For Syphilis, VDRL ICT kits were used and Malaria parasite was screen through MP slides. Blood grouping was performed by both forward and reverse methods
Results: This study showed a high frequency of HBsAg, VDRL and malaria positivity among the O-ve blood group donors, i.e. 3.70%, 9.25% and 0.61% respectively. Blood group B-ve individuals were commonly infected with HCV [12.5%] as compared with all other blood group donors. HIV is more commonly reported in A+ve blood group individuals. Blood group O+ve is more prevalent [37.41 %]
Conclusion: High frequency of HCV infection in blood donors advocates implementation of strict screening policy for donors and public awareness campaigns about preventive measures to reduce the spread of this infection as well as other transfusion transmissible infections
ABSTRACT
Objective: To determine the frequency of different fungal isolates involved in otomycosis along with their mode of presentation and outcome of treatment modalities
Study design: Descriptive Study
Place and Duration of study: Departments of E.N.T, Head and Neck Surgery and Department of Pathology, Liaquat University Hospital Hyderabad, from August 2014 to July 2016
Methodology: All patients of either age and gender who attended the E.N.T. outpatient department with the clinical diagnosis of otomycosis were included in the study
Results: A total of 164 patients were managed. There were 93 [56.7%] males and 71 [43.39%] females. Age ranged from 4-73 year with mean age of 35.33 year. Considering each ear as a separate entity, 125 [60.38%] sample ears were right sided and 82 [39.61%] left sided, with a total of 207 sample ears. The most common clinical feature in this study was blockage of the ear which was noted in 136 [65.70%] sample ears. The most common fungal specie isolated was Aspergillus niger [n= 56 - 27.05%]. Clotimazole 1% solution proved to be very effective to achieve clinical cure in 191[92.27%] sample ears within a short span of time
Conclusions: Otomycosis a disease of inner portion of external auditory canal was more common in males. The common predisposing factors were ear poking and use of antibiotics / steroid otic drops. Aspergillus Niger was the commonest specie isolated. Clotrimazole 1% solution proved to be effective for clinical cure followed by 2% salicylic acid in rectified spirit
ABSTRACT
To determine the frequency of Philadelphia chromosome [Ph] and its variants in chronic myeloid leukemia [CML] cases at a tertiary care hospital of Sindh. The study was conducted at the Department of Pathology, Liaquat University of Medical and Health Sciences, Jamshoro and Isra University Hospital, Hyderabad during May-to-September 2014. Bone marrow and peripheral blood samples from a total of 145 diagnosed cases of CML were collected. Cytogenetic analyses were performed using karyotyping as per the International System for Human Cytogenetic Nomenclature guidelines. All karyotypic images were analyzed using the Cytovision software. In order to identify BCR-ABL transcripts, RT-PCR was performed. Statistical analysis of the data was done using SPSS-version-21.0. Of the 145 samples, a total of 133 [91.7%] were positive for the Ph [Ph+] while 12 [8.3%] were negative for the Ph [Ph-]. Of the 133 Ph+ samples, standard karyotypes were noted in 121 [91%], simple variants in 9 [6.7%] and complex variants in 3 [2.3%] of the samples. All the Ph+ samples [n=133] showed BCR-ABL positivity. Of the 12 Ph- samples, a total of 7 [58.3%] were BCR-ABL-positive and 5 [41.6%] were BCR-ABL-negative. Frequency of the Ph was found to be of 90.9% in CML patients using a highly sensitive technique, the RT-PCR. Cytogenetic abnormalities were at a lower frequency. Cytogenetic and molecular studies must be conducted for better management of CML cases. These findings could be very useful in guiding the appropriate therapeutic options for CML patients
ABSTRACT
Objective: To determine the frequency of different sexual dysfuctions in type 2 diabetic males attending Diabetic OPD of Isra University Hospital and in private setup at Hyderabad
Design: Observational study
Duration of Study: March 2008 to August 2008
Methods: One-hundred type 2 diabetic males were enrolled by purposive sampling technique after obtaining a well informed consent. The different sexual dysfunctions were explained in the language best understood by the subject. Inclusion criteria was type 2 diabetic males of 35-65 years in age taking oral hypoglycemic agents for at least 5-years with HbA1c <9%. Patients with hypertensions, and any other endocrine disease like hypogonadism, thyroid and marked renal or hepatic insufficiency were excluded from the study. Frequencies, proportions and means with standard deviations are calculated for respective types of data
Results: Erectile dysfunction was most frequent, which was present in 55 patients, premature ejaculationin 20, hypoactive sexual desire disorder in 15 and retrograde ejaculation in 10 patients
Conclusion: Diabetes is associated with different sexual dysfunctions and most common is erectile dysfunction
ABSTRACT
Objective: to determine the predisposing factors of pregnancy induced hypertension in women of our community
Methods: an observational study was conducted at Gynecology and Obstetrics Department of Isra University Hyderabad from July 2007 to June 2008. Women with pregnancy induced hypertension were recruited in this study by convenient sampling from antenatal outpatient department, admitted patient in the ward and prelabour room. A restructured proforma was filled in which their age, BMI, parity, gestational age and gestational age when PIH [pregnancy induced hypertension] was diagnosed, regarding diabetes, multiple gestation, cardiac and liver disease, history of PIH in previous pregnancies and positive family history of PIH were recorded
Results: one hundred pregnant women were included in the study. We found 59% of women in age group of 30-40 years, 52% were overweight, 50% were multiparous and 15% were of grand-multiparous, 43% of women had history of PIH in previous pregnancies, 9% had positive family history and 12% were with diabetes. Twin pregnancy was seen in 3% , cardiac disease in 2% , renal disease in 1% , and hepatic disease in 1%
Conclusion: the observations of present study indicate that women between 30-years and 40-years of age, overweight [BMI >25-kg/m2], multipara, diabetic and who had history of PIH in previous pregnancies are at higher risk of developing pregnancy induced hypertension
ABSTRACT
Myocardial infarction is a major consequence of coronary artery disease. Recently many reports have been suggested that hyperhomocysteinemia had an important role in myocardial infarction. Plasma homocysteine level was determined in 60 myocardial infarction patient [Group A] and 35 healthy controls [Group B]. Statistically significant difference was observed in plasma homocystine concentrations between the patients of acute myocardial infarction [Group A] and in normal healthy individuals [Group B]. The level of homocystine in patients of myocardial infraction is significantly increased when compared with controls. This indicates a strong association between hyperhomocysteinemia and acute myocardial infraction in the peoples of Hyderabad, thus showing plasma homocysteine as a risk factor for myocardial infarction
Subject(s)
Humans , Male , Female , Homocysteine/blood , Hospitals, UniversityABSTRACT
The present study is designed to observe the effects of chronic cigarette smoking on lipid profile including triglycerides, total cholesterol, HDL-C, and LDL- C. A total of 102 [30 non-smokers and 72 smokers] apparently healthy male subjects with similar dietary habits and socioeconomic conditions were selected for this study. Total cholesterol, triglyceride and LDL-C were significantly increased in smokers [group B], when compared with non-smokers [Group A], while the level of HDL-C was significantly decreased in smokers as compared to the non-smokers. Total cholesterol, triglycerides and LDL-C were significantly increased in heavy smokers [Group B2] as compared to the moderate smokers [Group B1] and that of HDL-C was significantly decreased in heavy smokers as compared to moderate smokers
Subject(s)
Humans , Male , Cholesterol/blood , Triglycerides/blood , Lipoproteins, HDL/blood , Lipoproteins, HDL/bloodABSTRACT
Ninety subjects were included in the present study and were divided into three groups. Group A included 30 pregnant females with normal pregnancy as control from 20 weeks of gestation onward. Group B included 30 pregnant females with intrauterine fetal death < 20 weeks duration and Group C included 30 pregnant females with IUFD > 2 weeks duration. FDPs and D-dimers were performed by commercially available kits. Results were analysed by using chi-square [x2] test and level of significance was done. FDPs and D-dimers were significantly increased in females of IUFD in groups B and Crecommend it as a curative and palliative procedure for benign and malignant diseases of esophagus
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The present study was carried out to detect the serum anticardiolipin antibodies [ACA] in recurrent abortion. Fifty women with history of recurrent abortions [Group A] were selected with twenty normal women of childbearing age as controls [Group B]. Routine haematological investigations like haemoglobin, TLC and Platelets were done by haematology autoanalyzer. PT, APTT and serum anti-cardiolipin antibodies [IgG and IgM] were done by commercially available kits. Serum anticardiolipin antibodies were raised in patients with recurrent abortion when comparing with controls. PT was prolonged in one patient and APTT was prolonged in four cases
ABSTRACT
Sixty subjects were selected and were divided into two groups. Group A included 30 patients of end stage renal disease on regular dialysis and group B included 30 normal healthy subjects as control. Absolute values and Hb were done by hematology auto analyzer and serum and red cell folate were done by commercially available kits. Results were analyzed by using Student`s `t` test and level of significance was done. A significant decreased in serum and red cell folate in end stage renal disease patients with regular dialysis as compared to control
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A total of 80 full term pregnant women were selected and were divided into two groups on the basis of hemoglobin level. Group A included 40 pregnant women having Hb level more than 11.0 g/dl. Group A, included neonates born to group A mothers. Group B included 40 pregnant women having Hb < 11.0 g/dl and B, included their respective neonates. Serum iron, serum TIBC and serum ferritin were done by commercially available kits. Results obtained were analyzed by using students `t` test and level of significance was done. Serum iron and serum ferritin w as reduced in mothers of group B and their respective neonates while serum TIBC was higher in mothers and neonates of group B
ABSTRACT
Seventy Five subjects were selected. Fifty subjects were breast cancer patients and 25 healthy control subjects were included. FDPs and D-dimers were performed by using commercially available kits. Results obtained were analyzed by using chi-square [X2] test and level of significance was done. A significant increase in the levels of FDPs and D-dimers were found in patients with breast cancer